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Human IgG Isotype Control

Name: Human IgG Isotype Control
SKU: ABIN964452
Price: 309.36 EUR
Availability: InStock

ELISA, WB, SDS, DB IgG unconjugated Fc fragment

SDS-PAGE results of Human IgG F(c) Fragment. (Human IgG Isotype Control)

SDS

SDS-PAGE of Human IgG Fc. (Human IgG Isotype Control)

SDS

2 images

N° du produit ABIN964452

N° du produit (Fournisseur): 009-0103

Aperçu rapide pour Human IgG Isotype Control (ABIN964452)

Antigène

IgG

Fragment

Fc fragment

Application

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Dot Blot (DB)

Isotype

IgG

Hôte
137

127

124

83

82

80

64

55

46

42

38

33

33

32

25

18

16

10

8

7

3

2
Human

N° du produit (Fournisseur)

009-0103

Fournisseur

Rockland

Fonction

Human IgG Fc Fragment

Réactivité croisée (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.

Attributs du produit

Human Immunoglobulin G F(c) Fragment, IgG Fc

Purification

HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.

Stérilité

Sterile filtered
Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
IsoC, ELISA, IHC Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Indications d'application

Application Note: HUMAN IgG F(c) Fragment has been tested by SDS-Page and dot blot and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

1.029 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption

12 months
Antigène

IgG

Classe de substances

Antibody

Sujet

Background: Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.